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Catalog #1: bs-5774R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-5774R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-5774R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-6336R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Others

Conjugate: Biotin

Catalog #1: bs-6336R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Others

Conjugate: HRP

Catalog #1: bs-7800R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-7800R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bsm-0933M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-0933M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-10585R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10585R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Lane 1: Human Serum; Probed with RBP4 (3H7) Monoclonal Antibody (bsm-54054R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with RBP4 (3H7) Monoclonal Antibody (bsm-54054R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with RBP4 (3H7) Monoclonal Antibody (bsm-54054R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with RBP4 (3H7) Monoclonal Antibody (bsm-54054R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54054R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

A431 cells lysates probed with Lipocalin-2 (5C9) Monoclonal Antibody (bsm-52914R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Lipocalin-2 (5C9) Monoclonal Antibody (bsm-52914R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Lipocalin-2 (5C9) Monoclonal Antibody (bsm-52914R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human stomach cancer tissue incubated with Lipocalin-2 (5C9) Monoclonal Antibody (bsm-52914R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52914R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Human skeletal muscle lysate probed with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52557R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-0611r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0611r-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS-2 Polyclonal Antibody, Unconjugated (bs-20748R) at 1:400 overnight at 4\u00b0C, DAB staining.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IRS-2) polyclonal Antibody, Unconjugated (bs-20748R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with IRS-2 Polyclonal Antibody(bs-20748R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-20748R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-20748R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-20748R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-23362R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-23362R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-23362R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-22560R

Applications: IHC-P

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-22560R-Biotin

Applications: IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-22560R-HRP

Applications: IHC-P

Reactivity: Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (bs-22301R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (bs-22301R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRS1 Polyclonal Antibody, Unconjugated (bs-22301R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-22301R

Applications: IHC-P

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-22301R-Biotin

Applications: IHC-P

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-22301R-HRP

Applications: IHC-P

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0400r-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: HRP

Catalog #1: bs-0400r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Chicken

Conjugate: Biotin

Antigen: bs-6336P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n
Lane 1: Mouse Blood cell lysates probed with APOB48R Polyclonal Antibody, Unconjugated (bs-6336R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Spleen lysates probed with APOB48R Polyclonal Antibody, Unconjugated (bs-6336R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6336R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Others

Conjugate: Unconjugated

Catalog #1: bs-6336R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Others

Conjugate: Biotin

Catalog #1: bs-6336R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Mouse, Others

Conjugate: HRP

Catalog #1: bs-43518R

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43518R-Biotin

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-43518R-HRP

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-20436R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-20436R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-20436R-HRP

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-25117R-HRP

Applications: ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-25117R-Biotin

Applications: ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (GAD65) Polyclonal Antibody, Unconjugated (bs-25117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (GAD65) Polyclonal Antibody, Unconjugated (bs-25117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (GAD65) Polyclonal Antibody, Unconjugated (bs-25117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (GAD65) Polyclonal Antibody, Unconjugated (bs-25117R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-25117R

Applications: ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated