ZNT8 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZNT8 Polyclonal Antibody, Unconjugated (bs-22560R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ZNT8 Polyclonal Antibody, Unconjugated (bs-22560R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P

Reactivity

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bs-22560R
Product Name	ZNT8 Polyclonal Antibody
Applications	IHC-P
Reactivity	Rat
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ZNT8
Immunogen Range	1-100/369
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	169026
Swiss Prot	Q8IWU4
Subcellular location	Cytoplasm, Cell membrane
Synonyms	SLC30A8; solute carrier family 30 (zinc transporter), member 8; Solute carrier family 30 member 8; Zinc transporter 8; zinc transporter ZnT-8; ZnT-8; ZNT 8; ZNT8_HUMAN.
Background	ZNT8 is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010].
Application Dilution
IHC-P	1:200-400