Secondary-Free Western Blot? Why Direct-Labeled Primaries Are Worth a Try
This article compares direct-labeled primary antibodies with the traditional unlabeled primary antibody + labeled secondary antibody workflow for Western Blot detection. Direct-labeled primaries, such as HRP-conjugated antibodies, simplify the workflow by eliminating the secondary antibody step, which can reduce background, save time, improve reproducibility, and make multiplexing easier.