| Overview |
| bsm-70160M |
| Mitochondria Antibody |
| WB, IHC, ICC |
| Specific for endogenous levels of the ~60 kDa human mitochondria. The antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections. |
| Human |
| Specifications |
| Unconjugated |
| Mouse |
| Human cell homogenate |
| Monoclonal |
| 113-1 |
| IgG1 |
| Lot Dependent |
| Purified by Protein A. |
| PBS |
| Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. |
| Target |
| mitochondrial marker |
| Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998). This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. |
| Application Dilution |
| WB |
1:300-5000 |
| IHC |
|
| ICC |
1:100-500 |
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