Mitochondria Antibody

Overview
Catalog #	bsm-70160M
Product Name	Mitochondria Antibody
Applications	WB, IHC, ICC
Specificity	Specific for endogenous levels of the ~60 kDa human mitochondria. The antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections.
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Human cell homogenate
Clonality	Monoclonal
Clone #	113-1
Isotype	IgG1
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	PBS
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Target
Synonyms	mitochondrial marker
Background	Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998). This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Application Dilution
WB	1:300-5000
IHC
ICC	1:100-500