MeCP2 Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-70147M
Product Name	MeCP2 Antibody
Applications	WB, IHC, ICC
Specificity	Specific for endogenous levels of the MeCP2 protein. Due to usually high charge, the protein runs at ~75 kDa in SDS-PAGE of mouse brain lysates.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Full length human recombinant MeCP2
Clonality	Monoclonal
Clone #	5H12
Isotype	IgG2b
Concentration	Lot Dependent
Purification	Purified by Protein G.
Storage Buffer	PBS + 50% glycerol and 5 mM NaN3
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	4204
Swiss Prot	P51608
Synonyms	AUTSX 3 antibody, AUTSX3 antibody, DKFZp686A24160 antibody, Mbd 5 antibody, Mbd5 antibody, MECP 2 antibody, MeCP 2 protein antibody, MeCP-2 protein antibody, Mecp2 antibody, MECP2_HUMAN antibody, Methyl CpG binding protein 2 (Rett syndrome) antibody, Methyl CpG binding protein 2 antibody, Methyl-CpG-binding protein 2 antibody, MRX 16 antibody, MRX 79 antibody, MRX16 antibody, MRX79 antibody, MRXS 13 antibody, MRXS13 antibody, MRXSL antibody, PPMX antibody, RS antibody, RTS antibody, RTT antibody, WBP 10 antibody, WBP10 antibody
Background	MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Application Dilution
WB	1:300-5000
IHC
ICC	1:100-500