Phospho-ACACA (Ser80) Recombinant Antibody

$Western blot analysis of (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate. Using Phospho-ACACA (Ser80) (bsm-62566R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate. Using Phospho-ACACA (Ser80) (bsm-62566R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-62566r-100ul
Product Name	Phospho-ACACA (Ser80) Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Acetyl CoA carboxylase 1 around the phosphorylation site of S79
Modification Site	Ser80
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	107476
Swiss Prot	Q13085
Subcellular location	Cytoplasm
Synonyms	ACAC; ACACA; ACACB; ACC alpha; ACC; ACC beta; ACC1; ACC2; ACCA; ACCB; Acetyl CoA carboxylase 1; Acetyl CoA carboxylase 2;COA1; COA2.
Background	Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis.This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Application Dilution
WB	1:300-5000