Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody

$Lane 1: Daudi Cells; Lane 2: K562 Cells; Lane 3: Mouse Thymus; Lane 4: Mouse Kidney; Probed with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Daudi Cells; Lane 2: K562 Cells; Lane 3: Mouse Thymus; Lane 4: Mouse Kidney; Probed with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Seminal Vesicle tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Seminal Vesicle tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Daudi cells with Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody (bsm-54425R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54425R
Product Name	Methionine Aminopeptidase 2 (2G3) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within C-terminal human Methionine Aminopeptidase 2.
Clonality	Monoclonal
Clone #	2G3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	10988
Swiss Prot	P50579
Subcellular location	Cytoplasm
Synonyms	Methionine aminopeptidase 2; Initiation factor 2-associated 67 kDa glycoprotein; Peptidase M; MetAP 2; p67; p67eIF2; METAP2; MNPEP; P67EIF2
Background	Methionine aminopeptidases (MetAP), also designated peptidase M proteins, are members of the M24 family of proteins. Both MetAP-1 and MetAP-2 release N-terminal amino acids, usually methionine, from nascent peptides and arylamines. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. MetAP-1 and MetAP-2 control cell proliferation in mammalian cells. MetAP-2 is highly conserved between human and Saccharomyces cerevisiae. Neurofibromin (NF1) regulates MetAp-2 and increased expression of MetAP-2 correlates with several forms of cancer. Inhibitors of MetAP-2 are potential targets in cancer therapeutics, particularly in NF1-associated tumor proliferation. Chemotherapeutic drugs such as ovalicin and fumagillin bind to the active site of and inhibit MetAp-2.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200