CHAF1B (9A7) Monoclonal Antibody

$Lane 1: SiHa Cells; Lane 2: K562 Cells; Lane 3: A431 Cells; Probed with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SiHa Cells; Lane 2: K562 Cells; Lane 3: A431 Cells; Probed with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of K562 cells with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

$Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with CHAF1B (9A7) Monoclonal Antibody (bsm-54416R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-54416R
Product Name	CHAF1B (9A7) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein corresponding to the C-terminus of Human p60 CAF1.
Clonality	Monoclonal
Clone #	9A7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	8208
Swiss Prot	Q13112
Subcellular location	Cytoplasm, Nucleus
Synonyms	Chromatin assembly factor 1 subunit B; Chromatin assembly factor I p60 subunit; M-phase phosphoprotein 7; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60; CHAF1B; CAF1A; CAF1P60; MPHOSPH7; MPP7; p60 CAF1
Background	Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin-associated proteins 1 (HP1_, HP1_ and HP1_). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200
IP	1-2ug