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CHAF1B (9A7) Monoclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P
  • IF(IHC-P)
  • IF(ICC)
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-54416R
Product Name CHAF1B (9A7) Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein corresponding to the C-terminus of Human p60 CAF1.
Clonality Monoclonal
Clone # 9A7
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 8208
Swiss Prot Q13112
Subcellular location Cytoplasm, Nucleus
Synonyms Chromatin assembly factor 1 subunit B; Chromatin assembly factor I p60 subunit; M-phase phosphoprotein 7; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60; CHAF1B; CAF1A; CAF1P60; MPHOSPH7; MPP7; p60 CAF1
Background Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin-associated proteins 1 (HP1_, HP1_ and HP1_). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IP 1-2ug