GNL3 (6H5) Monoclonal Antibody

$IF(ICC) staining with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of A549 cells with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54389R
Product Name	GNL3 (6H5) Monoclonal Antibody
Applications	FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein corresponding to the C-terminus of human Nucleostemin.
Clonality	Monoclonal
Clone #	6H5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	26354
Swiss Prot	Q9BVP2
Subcellular location	Nucleus
Synonyms	Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47; Nucleolar GTP-binding protein 3; Nucleostemin; NNP47; GNL3; E2IG3;
Background	May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation. May interact with p53 and may be involved in tumorigenesis. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene
Application Dilution
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200