P2Y6 Recombinant Antibody

$Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: SK-BR-3 Cells; Lane 2: A549 Cells; Probed with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SK-BR-3 Cells; Lane 2: A549 Cells; Probed with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with P2Y6 (8H2) Monoclonal Antibody (bsm-54386R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54386r-100ul
Product Name	P2Y6 Recombinant Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide corresponding to N-terminal human P2Y6.
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	5031
Swiss Prot	Q15077
Subcellular location	Cell membrane
Synonyms	P2Y purinoceptor 6; P2Y6; P2RY6
Background	P2Y6 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. Four transcript variants encoding the same isoform have been identified for this gene. Receptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200