Nuf2 (4A3) Monoclonal Antibody

$Lane 1: K562 Cells; Lane 2: PC-3M Cells; Lane 3: THP-1 Cells; Probed with Nuf2 (4A3) Monoclonal Antibody (bsm-54346R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: K562 Cells; Lane 2: PC-3M Cells; Lane 3: THP-1 Cells; Probed with Nuf2 (4A3) Monoclonal Antibody (bsm-54346R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of A431 cells with Nuf2 (4A3) Monoclonal Antibody (bsm-54346R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

Reactivity

Human

Overview
Catalog #	bsm-54346R
Product Name	Nuf2 (4A3) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Nuf2 aa 1-200.
Clonality	Monoclonal
Clone #	4A3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	83540
Swiss Prot	Q9BZD4
Subcellular location	Nucleus
Synonyms	Kinetochore protein Nuf2; Cell division cycle-associated protein 1; hNuf2; hNuf2R; CDCA1; CT106
Background	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Application Dilution
WB	1:300-5000
FCM	1:20-100