Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody

Applications

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Bovine
Sheep
Non-Human Primate

Overview
Catalog #	bs-70229r-100ul
Product Name	Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Thr1007. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Bovine, Sheep, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1007 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Thr1007
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	171373
Swiss Prot	Q63633
Synonyms	Electroneutral potassium chloride cotransporter 2 antibody, Electroneutral potassium-chloride cotransporter 2 antibody, Erythroid K Cl cotransporter 2 antibody, Furosemide sensitive K Cl cotransporter antibody, hKCC2 antibody, K-Cl cotransporter 2 antibody, KCC 2 antibody, KCC2 antibody, KIAA1176 antibody, Neuronal K Cl cotransporter antibody, Neuronal K-Cl cotransporter antibody, Potassium Chloride Cotransporter antibody, Potassium chloride transporter 5 antibody, rKCC2 antibody, S12A5 antibody, S12A5_HUMAN antibody, SLC12A5 antibody, Solute carrier family 12 (potassium chloride transporter) member 5 antibody, Solute carrier family 12 member 5 antibody
Background	KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine (Lee et al., 2007). KCC2 is expressed in most adult neurons, and expression levels correlate well with the maturation state of neurons. N-ethylmaleimide (NEM) has been show to increase phosphorylation of the Ser-940 residue, while decreasing phosphorylation of Thr-1007 residue. Dephosphorylation of residues Thr-906 and Thr-1007 correlates with increased KCC2 activity (Moss et al., 2017).
Application Dilution
WB	1:300-5000