GABAA Receptor α4, Antibody

Applications

Reactivity

Mouse
Rat

Overview
Catalog #	bs-70099r-100ul
Product Name	GABAA Receptor α4, Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~ 64 kDa α4-subunit of the GABAA receptor.
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Fusion protein from the cytoplasmic loop of the α4 subunit of rat GABAA receptor.
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	140675
Swiss Prot	P28471
Synonyms	GABA(A) receptor subunit alphα4 antibody, GABA(A) receptor subunit alpha-4 antibody, GABR A4 antibody, GABRα4 antibodyGabra4 antibody, Gamma aminobutyric acid (GABA) A receptor alphα4 antibody, Gamma aminobutyric acid A receptor alphα4 antibody, Gamma aminobutyric acid receptor alphα4 subunit antibody, Gamma aminobutyric acid receptor subunit alphα4 antibody, Gamma-aminobutyric acid receptor subunit alpha-4 antibody, GBRA4_HUMAN antibody
Background	Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Application Dilution
WB	1:300-5000
IHC