GABAA Receptor α3 Antibody

Applications

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Bovine
Zebrafish
Non-Human Primate

Overview
Catalog #	bs-70098r-100ul
Product Name	GABAA Receptor α3 Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~51 kDa α3-subunit of the GABAA receptor. Immunolabeling is absent in α3-subunit knockout animals.
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Bovine, Zebrafish, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide from the N-terminal region of the α3 subunit of rat GABAA receptor.
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Gene ID	24947
Swiss Prot	P20236
Synonyms	GABA A Receptor alphα3 antibody, GABA(A) receptor subunit alphα3 antibody, GABA(A) receptor subunit alpha-3 antibody, GABR A3 antibody, GABRα3 antibody, Gabra3 antibody, Gamma aminobutyric acid (GABA) A receptor alphα3 antibody, Gamma aminobutyric acid A receptor alphα3 antibody, Gamma aminobutyric acid receptor subunit alphα3 antibody, Gamma-aminobutyric acid receptor subunit alpha-3 antibody, GBRA3_HUMAN antibody, MGC33793 antibody
Background	Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Application Dilution
WB	1:300-5000
IHC