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Primary Antibodies

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Catalog #1: bs-8996R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-8996R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Catalog #1: bs-8996R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-8996R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig

Conjugate: PE

Rat lung lysate 30ug, probed (bs-0061R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human spleen labeled with Rabbit Anti-eIF4ENIF1 Polyclonal Antibody, Unconjugated (bs-14561R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-eIF4ENIF1 Polyclonal Antibody, Unconjugated (bs-14561R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with eIF4ENIF1 Polyclonal Antibody, Unconjugated (bs-14561R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with eIF4ENIF1 Polyclonal Antibody, Unconjugated (bs-14561R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-14561R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Pig, Chimpanzee, Xenopus laevis

Conjugate: Unconjugated

Catalog #1: bs-14561R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chimpanzee, Xenopus laevis

Conjugate: Biotin

Catalog #1: bs-14561R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chimpanzee, Xenopus laevis

Conjugate: HRP

Catalog #1: bs-14561R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chimpanzee, Xenopus laevis

Conjugate: Cy5

Catalog #1: bs-14561R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chimpanzee, Xenopus laevis

Conjugate: Cy3

Catalog #1: bs-14561R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chimpanzee, Xenopus laevis

Conjugate: Cy7

Catalog #1: bs-14561R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chimpanzee, Xenopus laevis

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:300 overnight at 4\u00b0C, followed by Goat Anti-Mouse IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Cerebrum lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bs-33279M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Bax ) Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33279M

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33279M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bsm-33279M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bsm-33279M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bsm-33279M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bsm-33279M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bsm-33279M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Catalog #1: bs-20817R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat, Pig

Conjugate: HRP

Catalog #1: bs-20817R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat, Pig

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated \\tbs-20817R at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20817R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-20817R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Pig

Conjugate: Cy3

Catalog #1: bs-20817R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Pig

Conjugate: Cy5

Catalog #1: bs-20817R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Pig

Conjugate: Cy7

Catalog #1: bs-20817R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Pig

Conjugate: FITC

Catalog #1: bs-10900R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-10900R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-10900R) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse lung lysates;Lane 2: Mouse spleen lysates; Lane 3: Mouse thymus lysates probed with GAPDH-Loading Control Polyclonal Antibody, Unconjugated (bs-10900R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10900R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33033M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Lane 1: A549 Cell lysates; Lane 2: 293T Cell lysates; probed with GAPDH (4F8) Monoclonal Antibody, unconjugated (bsm-33033M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: A431 cell lysates; Lane 2: Hela cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Lovor cell lysates probed with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hyperpituitarism lysates probed with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Testis lysates; Lane 6: Rat Testis lysates; Lane 7: Mouse Kidney lysates; Lane 8: Human HUVEC cell lysates; Lane 9: Human A549 cell lysates; Lane 10: Human MCF-7 cell lysates probed with GAPDH Monoclonal Antibody, Unconjugated (bsm-33033M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33033M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33033M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-17422R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-17422R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

TT lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A431 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U251 lysates probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Stomach tissue probed with Histone H3.1 Polyclonal Antibody, Unconjugated (bs-17422R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-17422R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastritis labeled with Rabbit Anti TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U937 cells probed with Rabbit Anti-TREM2 Polyclonal Antibody, FITC Conjugated (bs-2723R-FITC) at 1:100 for 40 minutes (green) compared to control cells (blue).
Raje cell lysates probed with Anti-TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TREM2 Polyclonal Antibody, Unconjugated (bs-2723R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2723R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-23779R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-23779R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat, Pig

Conjugate: HRP

Mouse kidney lysates probed with SV2A Polyclonal Antibody, Unconjugated (bs-23779R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney lysates probed with SV2A Polyclonal Antibody, Unconjugated (bs-23779R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Spinal cord lysates probed with CPT1A Polyclonal Antibody, Unconjugated (bs-23779R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates probed with CPT1A Polyclonal Antibody, Unconjugated (bs-23779R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-23779R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33036M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Biotin

Lane 1: 293T; Lane 2: NIH\/3T3; Lane 3: mouse muscle lysates probed with beta-Actin (1A2) Monoclonal Antibody (bsm-33036M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lovo cell lysates probed with beta-Actin (1A2) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: A431 cell lysates probed with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Lung lysates probed with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33036M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bsm-33036M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP

Catalog #1: bs-11907R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Cy5.5

Catalog #1: bs-1206R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5.5

Catalog #1: bs-12418R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5.5

Catalog #1: bs-1294R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: Cy5.5

Catalog #1: bs-1324R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig, Guinea Pig

Conjugate: Cy5.5

Catalog #1: bs-1336R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep

Conjugate: Cy5.5