beta-Actin (1A2) Monoclonal Antibody

$Lane 1: 293T; Lane 2: NIH\/3T3; Lane 3: mouse muscle lysates probed with beta-Actin (1A2) Monoclonal Antibody (bsm-33036M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293T; Lane 2: NIH\/3T3; Lane 3: mouse muscle lysates probed with beta-Actin (1A2) Monoclonal Antibody (bsm-33036M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Lovo cell lysates probed with beta-Actin (1A2) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: A431 cell lysates probed with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: A431 cell lysates probed with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Lung lysates probed with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Lung lysates; Lane 4: Mouse Lung lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human A431 cell lysates; Lane 7: Rat Stomach lysates; Lane 8: Mouse Stomach lysates; Lane 9: Rat Cerebrum lysates; Lane 10: Mouse Cerebrum lysates probed with beta-Actin (1A2) Monoclonal Antibody, Unconjugated (bsm-33036M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Lung lysates; Lane 4: Mouse Lung lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human A431 cell lysates; Lane 7: Rat Stomach lysates; Lane 8: Mouse Stomach lysates; Lane 9: Rat Cerebrum lysates; Lane 10: Mouse Cerebrum lysates probed with beta-Actin (1A2) Monoclonal Antibody, Unconjugated (bsm-33036M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Mouse NIH\/3T3 cell lysates ; Lane 5: Human Hela cell lysates;Lane 6:Human A549 cell lysates; Lane 7: Mouse NIH\/3T3 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human A549 cell lysates; Lane 10: Mouse NIH\/3T3 cell lysates; Lane 11: Human Hela cell lysates ; Lane 12: Human A549 cell lysates probed with beta-Actin (1A2) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000~1:10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

$Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta-Actin (1A2) Monoclonal Antibody(bsm-33036M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta-Actin (1A2) Monoclonal Antibody(bsm-33036M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat
Bovine
Pig

Overview
Catalog #	bsm-33036M
Product Name	beta-Actin (1A2) Monoclonal Antibody
Applications	WB, IHC-P
Specificity	The immunogen of this antibody has sequence similarity to actin cytoplasmic 2 and beta-actin-like protein 2, and may bind to these targets.
Reactivity	Human, Mouse, Rat, Bovine, Pig
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human beta-Actin
Clonality	Monoclonal
Clone #	1A2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	60
Swiss Prot	P60709
Synonyms	Actin, cytoplasmic 1; ACTB
Background	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400