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Primary Antibodies

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Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IRS1 (Tyr632) ) polyclonal Antibody, Unconjugated (bs-8707R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-8707R

Applications: FCM, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-8707R-Biotin

Applications: IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-8707R-HRP

Applications: IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-8707R-Cy3

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy3

Catalog #1: bs-8707R-Cy5

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy5

Catalog #1: bs-8707R-Cy5.5

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-8707R-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy7

Catalog #1: bs-8707R-FITC

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: FITC

Catalog #1: bs-8707R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: PE

Catalog #1: bsm-33412M

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bsm-33412M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Biotin

Catalog #1: bsm-33412M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: HRP

Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Ctll-2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Bcl-2 monoclonal Antibody(bsm-33413M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33413M

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bsm-33413M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: HRP

Catalog #1: bsm-33413M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-21385R

Applications: IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse

Conjugate: Unconjugated

Catalog #1: bs-21385R-Biotin

Applications: IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse

Conjugate: Biotin

Catalog #1: bs-21385R-HRP

Applications: IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse

Conjugate: HRP

Catalog #1: bs-1640R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE

Catalog #1: bs-4727r-pe-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0042R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-3330R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: PE

Catalog #1: bs-3016R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PE

Catalog #1: bs-5551r-pe-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0061R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: PE

Catalog #1: bs-0439R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-10233R-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bs-1027r-pe-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PE

Catalog #1: bs-1050R-PE

Applications: WB, FCM

Reactivity: Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-1531R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE

Catalog #1: bs-3242R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-3737R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-4512R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0023R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-0023R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0023R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0023R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-0023R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-0501R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Chicken

Conjugate: PE

Catalog #1: bs-2235R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-18423R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-18423R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-18423R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-18423R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-18423R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-9439R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: PE

Catalog #1: bs-4609R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-4593R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: PE