RSPO1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RSPO1 Polyclonal Antibody, Unconjugated (bs-21385R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine
Dog
Pig
Rabbit
Horse

Overview
Catalog #	bs-21385R
Product Name	RSPO1 Polyclonal Antibody
Applications	IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RSPO1
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	284654
Swiss Prot	Q2MKA7
Subcellular location	Secreted
Synonyms	CRISTIN3; FLJ40906; hRspo1; R spondin homolog (Xenopus laevis); R spondin homolog; R spondin1; R-spondin-1; Roof plate specific spondin; Roof plate-specific spondin-1; RP11-566C13.1; RSPO; Rspo1; RSPO1_HUMAN.
Background	RSPO1 is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.
Application Dilution
IHC-P	1:200-400
IF(ICC)	IFICC1:50-200