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Primary Antibodies

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Catalog #1: bs-8747R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: HRP

Catalog #1: bs-8747R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Unconjugated

Catalog #1: bs-8747R-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy3

Catalog #1: bs-8747R-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy5

Catalog #1: bs-8747R-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy7

Catalog #1: bs-8747R-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy5.5

Catalog #1: bs-8747R-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: FITC

Formalin-fixed and paraffin embedded dog bladder labeled with Anti-Alpha-SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: dog bladder tissue labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated (bs-0189R) at 1:300, followed by conjugation to the secondary antibody.
Mouse Small Intestine labeled with Anti-Actin A\/Alpha SMA Polyclonal Antibody, Unconjugated(bs-0189R) 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C
NIH\/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with alpha Actin Polyclonal Antibody(bs-0189R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0189R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Unconjugated

Catalog #1: bs-10806R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Dog

Conjugate: Biotin

Lane 1: Mouse Heart lysates; Lane 2: Mouse Skin lysates; Lane 3: Mouse Thyroid gland lysates probed with NGFB Polyclonal Antibody, Unconjugated (bs-10806R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Brain Lysates; Lane 2: Mouse Cerebellum Lysates; Lane 3: Human U-87 MG cell Lysates; Lane 4: Human SH-SY5Y cell Lysates; Lane 5: Human U-2 OS cell Lysates. Probed with NGFB\u00a0polyclonal Antibody, unconjugated (bs-10806R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (bs-10806R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-10806R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-10806R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-10806R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-10806R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-10806R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-10806R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-10806R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-5551r-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

hepg2 lysates probed with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5551r-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-5551r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-5551r-cy3-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-5551r-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-5551r-fitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-5551r-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-5551r-cy55-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-9439R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Biotin

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-9439P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOXO1A Polyclonal Antibody(bs-9439R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Rat Lymph node lysates; Lane 2: Rat Bone lysates; Lane 3: Rat Liver lysates probed with FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9439R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Unconjugated

Catalog #1: bs-9439R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: HRP

Catalog #1: bs-9439R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy3

Catalog #1: bs-9439R-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy5.5

Catalog #1: bs-9439R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: FITC

Catalog #1: bs-9439R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy5

Catalog #1: bs-9439R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT2 (4G12) Monoclonal Antibody, Unconjugated (bsm-52221R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT2 (4G12) Monoclonal Antibody, Unconjugated (bsm-52221R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52221R

Applications: IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-20656R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDKN2A\/p16-INK4a Polyclonal Antibody, Unconjugated (bs-20656R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20656R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Dog

Conjugate: Unconjugated

Catalog #1: bs-20656R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: HRP

Catalog #1: bs-20656R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: Biotin

Catalog #1: bs-20656R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy3

Catalog #1: bs-20656R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy5

Catalog #1: bs-20656R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy5.5

Catalog #1: bs-20656R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: FITC

Catalog #1: bs-20656R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy7

Catalog #1: bs-18423R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-18423R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-18423R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-21401R-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit, Horse

Conjugate: Biotin

Catalog #1: bs-21401R

Applications: WB

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit, Horse

Conjugate: Unconjugated

Catalog #1: bs-21401R-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit, Horse

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD34 Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Testis tissue lysates probed with CD34 Monoclonal Antibody, Unconjugated (bsm-54470R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-54470R

Applications: FCM, IHC-P

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated