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Lane 1: Aphidicolin treated Mouse liver lysates; Lane 2: Untreated Mouse Liver lysates probed with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody (bsm-52159R) at 1:1000.
A549 cells were stained with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody (bsm-52159R) at 1:200 followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody (bsm-52159R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52159R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa lysates; Lane 2: NIH\/3T3 lysates; probed with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
A431 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
NIH\/3T3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT3(S727) (4G1 ) Monoclonal Antibody (bsm-52210R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52210R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates; probed with TrkB(Y817) (2A1 ) Monoclonal Antibody (bsm-52213R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
SH-SY-5Y cells were stained with TrkB(Y817) (2A1 ) Monoclonal Antibody (bsm-52213R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with TrkB (Tyr817) (2A1 ) Monoclonal Antibody (bsm-52213R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TrkB(Y817) (2A1) Monoclonal Antibody, Unconjugated (bsm-52213R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52213R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

293T Cell lysates; probed with AMPK alpha 1(S496) (11G1) Monoclonal Antibody (bsm-52132R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Human Hela cell lysates; Lane 2: Human Huvec cell lysates probed with AMPK alpha 1 (Ser496) (11G1) Monoclonal Antibody, Unconjugated (bsm-52132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52132R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8632R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Mouse cerebrum lysates probed with ONECUT2 Polyclonal Antibody, Unconjugated (bs-19643R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Jurkay lysates probed with ONECUT2 Polyclonal Antibody, Unconjugated (bs-19643R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-19643R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

K562 lysates probed with MAP3K13 Polyclonal Antibody, Unconjugated (bs-18663R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MAP3K13 Polyclonal Antibody (bs-18663R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-18663R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

DU145 lysates probed with JDP2 Polyclonal Antibody, unconjugated (bs-17200R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 lysates probed with JDP2 Polyclonal Antibody, unconjugated (bs-17200R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human DU145 cell lysates; Lane 5: Human HeLa cell lysates probed with JDP2 Polyclonal Antibody, Unconjugated (bs-17200R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-17200R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat cell lysates; Lane 2: Raji cell lysates; probed with CD31 (3B5) Polyclonal Antibody, unconjugated (bsm-10825M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Jurkat; Lane 2: U937; Lane 3: Raji cell lysates; probed with CD31 (3B5) Polyclonal Antibody, unconjugated (bsm-10825M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD31 (3B5) Monoclonal Antibody, Unconjugated (bsm-10825M) at 1:2000 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD31) Monoclonal Antibody, Unconjugated (bsm-10825M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-10825M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Fibronectin (1B9) Monoclonal Antibody (bsm-33143M) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Fibronectin (1B9) Monoclonal Antibody (bsm-33143M) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-33143M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2, Lane 2: Mouse cerebrum lysates probed with LTBR Polyclonal Antibody (bs-6917R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Rat Lymph node tissue lysates; Lane 3: Human A431 cell lysates probed with LTBR Polyclonal Antibody, Unconjugated (bs-6917R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6917R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Mouse Hippocampus lysates; Lane 2: Rat Liver lysates probed with Smad3 Polyclonal Antibody, Unconjugated (bs-19452R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Thr179) Polyclonal Antibody, Unconjugated (bs-19452R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Thr179) Polyclonal Antibody, Unconjugated (bs-19452R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Thr179) Polyclonal Antibody, Unconjugated (bs-19452R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-19452R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse lymph node lysate probed with CD8a Polyclonal Antibody (bs-10699R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Mouse Spleen cells probed with CD8a Polyclonal Antibody, unconjugated (bs-10699R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Rat Thymus tissue lysates probed with CD8 Polyclonal Antibody, Unconjugated (bs-10699R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10699R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse bladder lysates probed with GATA3 Polyclonal Antibody, Unconjugated (bs-23118R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse epithelium lysates probed with GATA3 Polyclonal Antibody, Unconjugated (bs-23118R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Rat Urinary bladder tissue lysates probed with GATA3 Polyclonal Antibody, Unconjugated (bs-23118R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-23118R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

HeLa cell lysate probed with CD163 Polyclonal Antibody (bs-23128R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Human Hela cell lysates; Lane 2: Human HL-60 cell lysates probed with CD163 Polyclonal Antibody, Unconjugated (bs-23128R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Lane 1: Human A375 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HL-60 cell lysates probed with CD163 Polyclonal Antibody, Unconjugated (bs-23128R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-23128R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53057R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Nuclear extracts from HeLa cells (40 \u03bcg) were analyzed by Western blot using the Bioss antibody against JMJD2a (Cat. No. bs-53136R) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of JMJD2a Polyclonal Antibody (bs-53136R), crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:9,000.

Catalog #1: bs-53136R

Applications: WB, ELISA, IF

Reactivity: Human

Conjugate: Unconjugated

To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human MBD4 (bs-53050R) in antigen-coated wells. The wells were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:15,000.
(Left) U2OS cells were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MBD4 (bs-53050R) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. (Right) Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 \u03bcg) with the antibody against MBD4 (bs-53050R), diluted 1:2,000 in TBST containing 3% milk powder.

Catalog #1: bs-53050R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53056R

Applications: ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Whole cell extracts (40 \u03bcg) from HeLa cells from an untransfected control (lane 1) and transfected with TBP siRNA (lane 2), and were analysed by Western blot using TBP Monoclonal Antibody (bsm-53003M) diluted 1:500 in TBSTween containing 5% skimmed milk, followed by secondary antibody.
ChIP assays were performed using U2OS cells, TBP (2C9) Monoclonal Antibody (bsm-53003M) and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 \u03bcg of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, and exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +\/- control target). These results demonstrate the occupancy of both promoters by TBP.

Catalog #1: bsm-53003M

Applications: WB, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Immunohistochemistry of TAF1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Primary antibody: TAF1 (bs-53045R) at 10 \u03bcg\/mL for 1h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 dilution for 45 min at RT. Staining: TAF1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Western blot using the TAF1 antibody (bs-53045R) to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3) and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown but may be degraded TAF1.

Catalog #1: bs-53045R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with ER alpha (5G7) Monoclonal Antibody (bsm-53007M) at a concentration of 7 \u03bcg\/ ml. Figure shows that the antibody is specific for ER alpha isoform, whereas the ER beta isoform is not recognized.
COS-7 cells transiently over-expressing human ER\u03b1 (left) or ER\u00df1 (right) were labeled with the ER alpha (5G7) Monoclonal Antibody (bsm-53007M), used at a concentration of 15 \u03bcg\/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure shows the specificity of the antibody for the ER alpha isoform.
ChIP assays was performed using MCF7 cells treated with the ER agonist estradiol for 3 hours prior to harvesting, ER alpha (5G7) Monoclonal Antibody (bsm-53007M), and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 \u03bcg of antibody were used per ChIP experiment. Recovery (%: ChIP\/input) and occupancy (x fold: +ve\/-ve) are shown. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +\/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.

Catalog #1: bsm-53007M

Applications: WB, IHC-P, IHC, dot-blot

Reactivity: Human, Monkey

Conjugate: Unconjugated

To determine the titer of the antibody, an ELISA was performed using a serial dilution MYH11 Polyclonal Antibody (bs-53091R). The plates were coated with the peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.
ChIP assays were performed using ME-1 cells, MYH11 Polyclonal Antibody (bs-53091R) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 \u03bcl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. The figure shows the relative occupancy, calculated as the ratio and control\/background for which the MYOG gene was used.
The IP\u2019d DNA from 6 ChIP\u2019s was pooled and subsequently analyzed on an Illumina HiSeq 2000. Library preparation, cluster generation, and sequencing were performed according to the manufacturer\u2019s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.

Catalog #1: bs-53091R

Applications: WB, ELISA, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53085R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human embryonic kidney cells (293T) were transfected with an ER-tagged PML\/RAR fusion protein. Nuclear extracts (50 \u03bcg) from control (lane 1) and from transfected cells (lane 2) were analyzed by Western blot using PML Polyclonal Antibody (Cat. No. bs-53134R). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The smaller band of approximately 60 kDa might represent the endogenous PML protein.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of PML Polyclonal Antibody (bs-53134R), crude serum and Flow-Through in antigen-coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:6,000.

Catalog #1: bs-53134R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-53084R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the DNMT1 antibody (bs-53048R), diluted 1:500 in BSA\/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right.
To determine the titer, an ELISA was performed using a serial dilution of the DNMT1 antibody (bs-53048R). The plates were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:9,600.

Catalog #1: bs-53048R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-53078R

Applications: ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H4K8ac Polyclonal Antibody (bs-53063R). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:17,500.
A Dot Blot analysis was performed to test the cross-reactivity of \r\nH4K8ac Polyclonal Antibody (bs-53063R) with peptides containing other modifications of histone H4 and H3 and an unmodified histone H4 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.
Histone extracts of HeLa cells (15 \u03bcg) were analyzed by Western blot using the antibody against H4K8ac (bs-53063R) diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right. Lane 2 shows the result of the Western analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature.
ChIP assays were performed using HeLa cells treated with colcemid, H4K8ac Polyclonal Antibody (bs-53063R) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 10,000 cells. A titration of the antibody consisting of 0.5, 1.5, and 5 \u03bcl per ChIP experiment was analyzed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes ALDOA and MYOD. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53063R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated

HeLa cells were stained with the Bioss antibody against HP1\u03b1 (Cat. No. bs-53123R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the HP1\u03b1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Western blot was performed on nuclear extracts from HeLa cells (20 \u03bcg) with the Bioss antibody against human HP1\u03b1 (Cat. No. bs-53123R), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the expected location of HP1\u03b1, HP1\u03b2, and HP1\u03b3 is indicated on the right.

Catalog #1: bs-53123R

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

HeLa cells were stained with the Bioss antibody against WDR5 (Cat. No. bs-53101R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the WDR5 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Nuclear extracts from HeLa cells (20 \u03bcg) were analyzed by Western blot using the Bioss antibody against WDR5 (Cat. No. bs-53101R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 \u03bcg of the Bioss antibody against WDR5 (Cat. No. bs-53101R) as described above. The IP\u2019d DNA was subsequently analyzed on an Illumina HiSeq. Library preparation, cluster generation, and sequencing were performed according to the manufacturer\u2019s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. The figure shows the enrichment along the complete sequence and a 2 Mb region of human chromosome 1 (fig A and B), and in two genomic regions surrounding the RPL41 and RPS4X positive control genes (fig C and D).
ChIP assays were performed using HeLa cells, the Bioss antibody against WDR5 (Cat. No. bs-53101R) and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 \u03bcg of antibody per ChIP experiment was analyzed. IgG (2 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the RPL41 and RPS4X ribosomal protein genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53101R

Applications: WB, IF, ChIP-seq

Reactivity: Human

Conjugate: Unconjugated

Histone extracts of HeLa cells (15 \u03bcg) were analysed by Western blot using the H4K20me1 Polyclonal Antibody (bs-53035R) diluted 1:750 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
To check the specificity of H4K20me1 Polyclonal Antibody (bs-53035R) at 1:20,000 dilution, a Dot Blot was performed with peptides containing different modifications of histone H4 and the unmodified sequence. 0.2 to 100 pmol concentration of peptides were spotted on to a membrane. Experiment shows specificity of the antibody for the modification of interest.
To determine the titer, an ELISA was performed using a serial dilution of the H4K20me1 Polyclonal Antibody (bs-53035R), to detect peptide containing histone modification of interest. By plotting the absorbance against antibody dilutions, titer of the antibody was estimated to be 1:8,000.
ChIP were performed using U2OS cells using H4K20me1 Polyclonal Antibody (bs-53035R). Chromatin was sheared from 1.6 million cells. A titration of the antibody consisting of 2, 5, 10 and 15\u03bcl per ChIP experiment was analyzed. IgG (5\u03bcg\/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for the coding region of the myogenic differentiation gene (MYOD) which is inactive at normal conditions. This figure shows the recovery, expressed as a % of input.

Catalog #1: bs-53035R

Applications: WB, ELISA, dot-blot

Reactivity: Human, Others

Conjugate: Unconjugated

Western blot was performed on whole cell extracts (25 \u03bcg, lane 1) and histone extracts (15 \u03bcg, lane 2) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4pan Polyclonal Antibody(bs-53138R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H4pan Polyclonal Antibody (bs-53138R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H4 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of H4pan Polyclonal Antibody (bs-53138R) in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,000.
ChIP assays were performed using HeLa cells, H4pan Polyclonal Antibody (bs-53138R) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells on the IP-Star Compact automated system. A titration consisting of 0.5, 1, 2 and 5 \u03bcg of antibody per ChIP experiment was analyzed. IgG (1 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and c-fos genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53138R

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Mouse NIH3T3 cells were stained with Bioss antibody against H4K5,8,12,16ac (cat. No. bs-53098R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K5,8,12,16ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the cross-reactivity of the Bioss antibody against H4K5,8,12,16ac (cat. No. bs-53098R), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K5,8,12,16ac (cat. No. bs-53098R) in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,200.
ChIP was performed with 2 \u03bcg of the Bioss antibody against H4K5,8,12,16ac (cat. No. bs-53098R) on sheared chromatin from 1 million HeLa cells using a ChIP-seq kit as described above. The IP\u2019d DNA was subsequently analyzed on an Illumina Genome Analyzer. Library preparation, cluster generation, and sequencing were performed according to the manufacturer\u2019s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure shows the signal distribution along the complete length of chromosome 5 (figure A) and a zoomin to a 600 kb region (figure B). Figure C and D show the enrichment in two genomic regions on chromosome 3 and 12, respectively, containing EIF4A2 and GAPDH positive controls. The position of the amplicon used for validating the QPCR results is shown with an arrow.

Catalog #1: bs-53098R

Applications: ELISA, IF, dot-blot, ChIP-seq

Reactivity: Human, Mouse

Conjugate: Unconjugated

Western blot was performed on whole cell (25\u03bcg, lane 1) and histone extracts (15\u03bcg, lane 2) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4K5ac Polyclonal Antibody (bs-53099R). Antibody was diluted 1:500 in TBS-Tween containing 5% skim milk.
Dot Blot analysis was performed with peptides containing a variety of histone modifications and the unmodified H4. 0.2-100 pmol of the respective peptides were spotted on a membrane. H4K5ac Polyclonal Antibody (bs-53099R) was used at 1:5,000 dilution. Result shows high specificity of the antibody for the modification of interest.
ChIP were performed using HeLa cells using H4K5ac Polyclonal Antibody (bs-53099R). Chromatin was sheared from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10\u03bcg per ChIP experiment was analyzed. IgG (2\u03bcg\/IP) was used as negative IP control. Quantitative PCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH as positive controls, and for the inactive MYOD1 gene and Sat2 satellite repeat region used as negative controls. Result shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ELISA was performed using a serial dilution of the\r\nH4K5ac Polyclonal Antibody (bs-53099R) against peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,900.

Catalog #1: bs-53099R

Applications: WB, ELISA, IHC-P, IF(ICC), dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

HeLa cells were stained with the Bioss antibody against H4K20me1 (Cat. bs-53104R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K20me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Western blot was performed on whole cell extracts (25 \u03bcg, lane 1) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the Bioss antibody against H4K20me1 (cat. No. bs-53104R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right.
To check the specificity of the Bioss antibody against H4K20me1 (cat. No bs-53104R) a Dot Blot was performed with peptides containing different modifications of histone H3 and H4 or the unmodified H4K20 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.
To determine the titer, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K20me1 (cat. No. bs-53104R) in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:51,100.

Catalog #1: bs-53104R

Applications: WB, ELISA, IF(ICC), dot-blot

Reactivity: Human

Conjugate: Unconjugated

A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H4K20me3 (Cat. No. bs-53115R) with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest
Human osteosarcoma (U2OS) cells were stained with the Bioss antibody against H4K20me3 (Cat. No. bs-53115R) and with DAPI. Cells were fixed with ice-cold methanol for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H4K20me3 antibody (left) diluted 1:300 in blocking solution followed by an anti-rabbit secondary antibody conjugated to Alexa568 or with DAPI (right). Figure B: staining of the cells with the H4K20me3 antibody after incubation of the antibody with blocking peptide at 5 ng\/\u03bcl.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K20me3 (Cat. No. bs-53115R), crude serum and flow through in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:7,400.
ChIP assays were performed using HeLa cells, the Bioss antibody against H4K20me3 (Cat. No. bs-53115R) and optimized PCR primer sets for qPCR. ChIP was performed with an Auto Histone ChIP-seq kit with sheared chromatin from 1 million cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 \u03bcg per ChIP experiment was analyzed. IgG (1 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for promoters of the active genes c-fos and GAPDH, used as negative controls, and for the Sat2 satellite repeat region used as a positive control. The figure shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53115R

Applications: WB, ELISA, IHC-P, IF(ICC), IF, dot-blot, ChIP-seq

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

A Dot Blot analysis was performed to test the cross-reactivity of the Bioss antibody against H4K8ac (cat. No. bs-53133R) with peptides containing other histone modifications and the unmodified H4K8. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000.
Histone extracts of HeLa cells (15 \u03bcg) were analyzed by Western blot using the Bioss antibody directed against H4K8ac (cat. No. bs-53133R) diluted 1:200 in TBS-Tween containing 5% skimmed milk.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K8ac (cat. No. bs-53133R), crude serum and flow through in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:16,700.
Mouse NIH3T3 cells were stained with H4K8ac Polyclonal Antibody (cat. No. bs-53133R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K8ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Catalog #1: bs-53133R

Applications: WB, ELISA, IF, dot-blot

Reactivity: Human, Mouse

Conjugate: Unconjugated

HeLa cells were stained with the Bioss antibody against H4K5,8,12ac (cat. No. bs-53096R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. Figure A: cells were immunofluorescently labeled with the H4K5,8,12ac antibody (left) diluted 1:500 in blocking solution followed by Alexa488 conjugated secondary antibody. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Figure B, C, D, and E: staining of the cells with the H4K5,8,12ac antibody after incubation of the antibody with 10 ng\/\u03bcl of the following blocking peptides: H4K5,8,12 unmodified (figure B), H4K5,8,12ac (figure C), H2A.ZK5,7,11ac (figure D) and H4K5,8,12,16ac (figure E).
Western blot was performed on whole cell (25 \u03bcg, lane 1) and histone extracts (15 \u03bcg, lane 2) of HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Bioss antibody against H4K5,8,12ac (cat. No. bs-53096R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
To test the cross-reactivity of the Bioss antibody against H4K5,8,12ac (cat. No. bs-53096R), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against H4K5,8,12ac (cat. No. bs-53096R) in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.

Catalog #1: bs-53096R

Applications: WB, ELISA, IP, IF, dot-blot, ChIP-seq

Reactivity: Human, Mouse

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4un antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4un (3C5) Monoclonal Antibody (Cat. No. bsm-53012M). The wells were coated with peptides containing the unmodified H3K4 region as well as the mono-, di- and trimethylated H3K4. Figure 2 shows a high specificity of the antibody for the unmodified peptide.
ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K4un (Cat. No. bsm-53012M) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analysed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53012M

Applications: WB, ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-53009M

Applications: WB, dot-blot

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K4me1 Monoclonal Antibody (bsm-53013M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the molecular weight marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using HeLa cells, H3K4me1 (34A1) Monoclonal Antibody (bsm-53013M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analyzed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53013M

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Western blot was performed on whole cell extracts from HeLa cells (25 \u03bcg, lane 1) and recombinant histone H2A, H2B, H3 and H4 (1ug, lane 2, 3, 4 and 5, respectively) using the H3pan (1B2) Monoclonal Antibody (bsm-53008M). The antibody was diluted 1:1,000 in TBSTween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
Western blot was performed on whole cell extracts (30\u03bcg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using H3pan Monoclonal Antibody (bsm-53008M) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
HeLa cells stained with the H3pan Monoclonal Antibody (bsm-53008M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 1% BSA. The cells were labeled with the H3 antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Catalog #1: bsm-53008M

Applications: WB, IF(ICC)

Reactivity: Human, Others

Conjugate: Unconjugated

Western blot was performed on whole cell (25 \u03bcg, lane 1) and histone extracts (15 \u03bcg, lane 2) from HeLa cells, and on 1 \u03bcg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H3K9un (7D7) Monoclonal Antibody (bsm-53024M). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K9un (7D7) Monoclonal Antibody (bsm-53024M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9un antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
ChIP assays were performed using HeLa cells, H3K9un (7D7) Monoclonal Antibody (bsm-53024M) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 1 million cells, using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 \u03bcg per ChIP experiment was analysed. IgG (2 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the coding regions of the MYOD1 and MB genes and for a region 1 kb upstream of the GAPDH promoter, used as positive controls, and for the ZNF510 coding region, the EIF4A2 promoter and the Sat2 satellite repeat region, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53024M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K4me2 (4B4) Monoclonal Antibody (cat. No. bsm-53014M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4me2 (4B4) Monoclonal Antibody (cat. No. bsm-53014M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two staining is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of the H3K4me2 (4B4) Monoclonal Antibody (cat. No. bsm-53014M). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the dimethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using HeLa cells, H3K4me2 (4B4) Monoclonal Antibody (cat. No. bsm-53014M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP was analysed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53014M

Applications: WB, ELISA, IF(ICC)

Reactivity: Human, Others

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K4me3 (2G10) Monoclonal Antibody (Cat. No. bsm-53015M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4me3 (2G10) Monoclonal Antibody (Cat. No. bsm-53015M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two staining is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4me3 (2G10) Monoclonal Antibody (Cat. No. bsm-53015M). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using human HeLa cells, \r\nH3K4me3 (2G10) Monoclonal Antibody (bsm-53015M) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells on the SX-8G IP-Star automated system. A titration consisting of 1, 2, 5 and 10 \u03bcg of antibody per ChIP experiment was analyzed. IgG (2 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of GAPDH and c-fos genes (positive controls), and exon 2 of the inactive myoglobin (MB) gene and Sat2 satellite repeat (negative controls). Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.

Catalog #1: bsm-53015M

Applications: WB, ELISA, IF(ICC)

Reactivity: Human, Others

Conjugate: Unconjugated

To test the specificity an ELISA was performed using a serial dilution using 3K36me2 (6C9) Monoclonal Antibody (bsm-53020M). The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.
ChIP assays were performed using HeLa cells, H3K36me2 (6C9) Monoclonal Antibody (bsm-53020M) and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 \u03bcg of antibody per ChIP experiment was analyzed. IgG (1 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53020M

Applications: ELISA, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using H3K9me2 (9B3) Monoclonal Antibody (Cat. No. bsm-53016M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
HeLa cells were stained with H3K9me2 (9B3) Monoclonal Antibody (bsm-53016M). Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
To test the specificity an ELISA was performed using a serial dilution H3K9me2 (9B3) Monoclonal Antibody (bsm-53016M). The wells were coated with peptides containing the unmodified H3K9 as well as the mono-, di- and trimethylated H3K9 and the dimethylated H3K4 and H3K27. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using HeLa cells, H3K9me2 (9B3) Monoclonal Antibody (bsm-53016M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 3 and 9 \u03bcg per ChIP experiment was analysed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53016M

Applications: WB, ELISA, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated