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Primary Antibodies

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Catalog #1: bsm-33210M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-33210M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Lane 1: BV2 Cell lysates; Lane 2: VSC4.1 Cell lysates; probed with GAP43 (6F11) Monoclonal Antibody, unconjugated (bsm-33192M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GAP43 (6F11) Monoclonal Antibody (bsm-33192M) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GAP43 (6F11) Monoclonal Antibody (bsm-33192M) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAP43 (6F11) Monoclonal Antibody, Unconjugated (bsm-33192M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33192M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

293T Cell lysates; probed with STAT3 (3F5) Monoclonal Antibody, unconjugated (bsm-33218M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A594 Cell lysates; probed with STAT3 (3F5) Monoclonal Antibody, unconjugated (bsm-33218M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 Cell lysates; probed with STAT3 (3F5) Monoclonal Antibody, unconjugated (bsm-33218M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT3 (3F5) Monoclonal Antibody, Unconjugated (bsm-33218M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33218M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

NIH\/3T3 cell lysates probed with CREB1 (6B4) Monoclonal Antibody, unconjugated (bsm-33196M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CREB1 (6B4) Monoclonal Antibody, Unconjugated (bsm-33196M) at 1:2000 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Jurkat cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U-2OS cell lysates probed with CREB1 (6B4) Monoclonal Antibody, Unconjugated (bsm-33196M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33196M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: HeLa cell lysates; Lane 2: NIH\/3T3 Cell lysates; probed with HSP90A (3H7) Monoclonal Antibody, unconjugated (bsm-33210M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with HSP90A (3H7) Monoclonal Antibody (bsm-33210M) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with HSP90A (3H7) Monoclonal Antibody (bsm-33210M) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Placenta tissue lysates ; Lane 5: Human U251 cell lysates ; Lane 6: Human Molt-4 cell lysates ; Lane 7: Human HL60 cell lysates ; Lane 8: Human A549 cell lysates probed with HSP90 alpha Monoclonal Antibody, Unconjugated (bsm-33210M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33210M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33218M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33218M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

HeLa cells were stained with MSK1 (Ser376) (11A1) Monoclonal Antibody (bsm-52123R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
PC-3 cells were stained with MSK1 (Ser376) (11A1) Monoclonal Antibody (bsm-52123R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: HL60 cell lysates probed with MSK1 (Ser376) (11A1) Monoclonal Antibody, Unconjugated (bsm-52123R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Rat bladder tissue incubated with MSK1 (Ser376) (11A1) Monoclonal Antibody (bsm-52123R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52123R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52011R

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52070R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52042M

Applications: WB

Reactivity: Human, Others

Conjugate: Unconjugated

HepG2 cells were stained with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF-7 cells were stained with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Cyclin E1 (4H7) Monoclonal Antibody (bsm-52048R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52048R

Applications: IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Collagen XVII (1C11) Monoclonal Antibody (bsm-52041R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen 17 (1C11) Monoclonal Antibody, Unconjugated (bsm-52041R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen 17 (1C11) Monoclonal Antibody, Unconjugated (bsm-52041R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Collagen 17 (1C11) Monoclonal Antibody, Unconjugated (bsm-52041R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52041R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa cell lysates probed with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human kidney section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
NIH\/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52015R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Jurkat; Lane 2: Raji lysates probed with IRF7 (2A1) Monoclonal Antibody (bsm-52120R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
293 cells were stained with IRF7 (2A1) Monoclonal Antibody (bsm-52120R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: Human Jurkat cell lysates; Lane 2: Human Raji cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human MOLT-4 cell lysates; Lane 6: Human HUVEC cell lysates probed with IRF7 (2A1) Monoclonal Antibody, Unconjugated (bsm-52120R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52120R

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

293T cell lysates probed with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
HeLa cells were stained with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF-7 cells were stained with Cytokeratin 4 (2F9) Monoclonal Antibody (bsm-52062R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52062R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52040R

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293 lysates; Lane 2: Mouse lung lysates probed with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
MCF-7 cells were stained with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:200 dilution, incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: Human MCF-7 cell Lysates; Lane 2: Human HepG2 cell Lysates. Probed with FOXA1 monoclonal Antibody, unconjugated (bsm-52072R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52072R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat cell lysate probed with BCL-2 (2C4) Monoclonal Antibody, Unconjugated (bsm-52022M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with BCL-2 (2C4) Monoclonal Antibody (bsm-52022M) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52022M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Jurkat cell lysate probed with ADAM17 (9E7) Monoclonal Antibody, Unconjugated (bsm-52009R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
SK-OV-3 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:200 in blocking solution. Slides were incubated overnight at 4\u00b0C followed by Cy5 conjugated secondary antibody incubation and DAPI staining of the nucleus.
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52009R

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: U937; Lane 2: Raji cell lysate probed with PIK3R1 (9B3) Monoclonal Antibody (bsm-52219M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
MCF7 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with PIK3R1 (9B3) Monoclonal Antibody (bsm-52219M) at 1:200 in blocking solution. Slides were incubated overnight at 4\u00b0C followed by FITC conjugated secondary antibody inclubation and DAPI staining of the nucleus.

Catalog #1: bsm-52219M

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: NIH\/3T3 lysates; Lane 2: Hela lysates probed with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Hela cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT6 (9A7) Monoclonal Antibody at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52239R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human lung lysates probed with IRF2 (5F2) Monoclonal Antibody (bsm-52115R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
A549 cells were stained with IRF2 (5F2) Monoclonal Antibody (bsm-52115R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52115R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: PC-12 Cell lysates; Lane 2: Jurkat Cell lysates; probed with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
NIH\/3T3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:100 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52114R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

HeLa cell lysate probed with Cdk4 (2F6) Monoclonal Antibody, Unconjugated (bsm-52028M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cdk4 (2F6) Monoclonal Antibody, Unconjugated (bsm-52028M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cdk4 (2F6) Monoclonal Antibody, Unconjugated (bsm-52028M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cdk4 (2F6) Monoclonal Antibody, Unconjugated (bsm-52028M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52028M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: K562 cell lysates; probed with STAT5a (6E5) Monoclonal Antibody (bsm-52237R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT5a (6E5) Monoclonal Antibody (bsm-52237R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
HeLa cells were stained with STAT5a (6E5) Monoclonal Antibody (bsm-52237R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52237R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human lung lysates probed with Cytokeratin 13 (5A3) Monoclonal Antibody(bsm-52053R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 13 (5A3) Monoclonal Antibody (bsm-52053R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A549 cells were stained with Cytokeratin 13 (5A3) Monoclonal Antibody (bsm-52053R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF-7 cells were stained with Cytokeratin 13 (5A3) Monoclonal Antibody (bsm-52053R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52053R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: PC-12 Cell lysates; Lane 3: CRC lysates; probed with ERK2 (3G1) Monoclonal Antibody (bsm-52068R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with ERK2 (3G1) Monoclonal Antibody (bsm-52068R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with ERK2 (3G1) Monoclonal Antibody (bsm-52068R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: K562 cell lysates; Lane 2: Siha cell lysates probed with ERK2 (3G1) Monoclonal Antibody, Unconjugated (bsm-52068R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52068R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T Cell lysates; Lane 2: K562 lysates; probed with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
SHG-44 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52082R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

K562 lysates probed with IRF6 (1A1) Monoclonal Antibody (bsm-52119R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with IRF6 (1A1) Monoclonal Antibody (bsm-52119R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF6 (1A1) Monoclonal Antibody, Unconjugated (bsm-52119R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF6 (1A1) Monoclonal Antibody, Unconjugated (bsm-52119R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52119R

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: JAR lysates; Lane 2: HUVEC lysates; Lane 3: Hela lysates probed with Alas1 (1G11) Monoclonal Antibody (bsm-52012R) at 1:1000.
HepG2 cells were stained with Alas1 (1G11) Monoclonal Antibody (bsm-52012R) at 1:200 in PBS and incubated for overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection
Lane 1: Human JAR cell lysates; Lane 2: Human Huvec cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human HepG2 cell lysates ; Lane 5: Human MCF-7 cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human Raji cell lysates probed with Alas1 (1G11) Monoclonal Antibody, Unconjugated (bsm-52012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52012R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

HeLa cell lysates probed with ERK5 (8D8) Monoclonal Antibody (bsm-52069R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
MCF-7 cells were stained with ERK5 (8D8) Monoclonal Antibody (bsm-52069R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ERK5 (8D8) Monoclonal Antibody(bsm-52069R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed and paraffin-embedded Rat testis tissue incubated with ERK5 (8D8) Monoclonal Antibody (bsm-52069R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52069R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Jurkat; Lane 2: Hela lysates probed with Cyclin D3 (4A8) Monoclonal Antibody (bsm-52047R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
A431 cells were stained with Cyclin D3 (4A8) Monoclonal Antibody (bsm-52047R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: Human MOLT-4 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Panc-1 cell lysates probed with Cyclin D3 Monoclonal Antibody, Unconjugated (bsm-52047R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52047R

Applications: WB, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with BCL2A1 (3E4) Monoclonal Antibody (bsm-52023R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with BCL2A1 (3E4) Monoclonal Antibody (bsm-52023R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52023R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat cell lysates; probed with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with CDK1 (3E12) Monoclonal Antibody (bsm-52026R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52026R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

MCF-7 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52050R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: NIH\/3T3; Lane 3: PC12 lysates probed with Histone H2B (3A6) Monoclonal Antibody (bsm-52099R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
A431 cells were stained with Histone H2B (3A6) Monoclonal Antibody (bsm-52099R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Histone H2B (3A6) Monoclonal Antibody (bsm-52099R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Histone H2B (3A6) Monoclonal Antibody (bsm-52099R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52099R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Histone H4 (2G2) Monoclonal Antibody (bsm-52111R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Histone H4 (2G2) Monoclonal Antibody (bsm-52111R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse spleen lysate probed with Histone H4 (2G2) Monoclonal Antibody (bsm-52111R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .

Catalog #1: bsm-52111R

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human skin lysates; probed with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
A431 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 16 (1A4) Monoclonal Antibody (bsm-52056R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52056R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 17 (1B12) Monoclonal Antibody (bsm-52057R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
PC-3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 17 (1B12) Monoclonal Antibody (bsm-52057R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52057R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: Jurkat Cell lysates; Lane 3: K562 cell lysates; probed with HDAC1 (3C3) Monoclonal Antibody (bsm-52080R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with HDAC1 (3C3) Monoclonal Antibody (bsm-52080R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human U251 cell lysates probed with HDAC1 Monoclonal Antibody, Unconjugated bsm-52080R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52080R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

THP-1 cell lysates probed with IRF5 (3F11) Monoclonal Antibody (bsm-52117R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with IRF5 (3F11) Monoclonal Antibody (bsm-52117R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF5 (3F11) Monoclonal Antibody, Unconjugated (bsm-52117R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF5 (3F11) Monoclonal Antibody, Unconjugated (bsm-52117R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52117R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52124R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 cells were fixed in paraformaldehyde and permeabilized with 0.25% Triton X100\/PBS. Antibody incubation with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAPI for nuclei staining
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue was incubated with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counter stained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue was incubated with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue was incubated with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counter stained with hematoxylin

Catalog #1: bsm-52234R

Applications: IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa lysates; Lane 2: SHG-44 lysates; Lane 3: SW480 Lysates; probed with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
CRC cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Stat3 Antibody(bsm-52235R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-52235R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin B1 (2E6) Monoclonal Antibody (bsm-52044R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u2103\r\novernight\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Jurkat, 2: HeLa, 3: HT-29\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 55 kDa\r\nObserved MW: 55 kDa

Catalog #1: bsm-52044R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Hela lysates; Lane 2:A431 lysates; Lane 3: PC12 lysates probed with Smad2 (9A3) Monoclonal Antibody (bsm-52223R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Hela cells were stained with Smad2 (9A3) Monoclonal Antibody (bsm-52223R) at [1:200] incubated for overnight at 4C followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad2 (9A3) Monoclonal Antibody(bsm-52223R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: MouseTestis lysates; Lane 2: Human A431 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HL60 cell lysates; Lane 5: Human Jurkat cell lysates probed with Smad2 monoclonal Antibody, Unconjugated (bsm-52223R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52223R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated