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Primary Antibodies

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Catalog #1: bsm-51428M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: T47D Cell lysates; probed with ALDH6A1 (147CT8.3.4) Monoclonal Antibody, unconjugated (bsm-51430M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ALDH6A1 (147CT8.3.4) Monoclonal Antibody (bsm-51430M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ALDH6A1 (147CT8.3.4) Monoclonal Antibody (bsm-51430M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51430M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; Lane 2: SK-BR-3 Cell lysates; Lane 3: C2C12 Cell lysates; probed with CAPN2 (1C1) Monoclonal Antibody, unconjugated (bsm-51429M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN2 (1C1) Monoclonal Antibody (bsm-51429M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Flow cytometric analysis of U-87 MG cells using bsm-51429M CAPN2 Antibody (green) compared to mouse IgG2b isotype control (blue). bsm-51429M was diluted at 1:100 dilution. Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Catalog #1: bsm-51429M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51431M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: HeLa; Lane 2: THP-1; Lane 3: A431 cell lysate at 20 \u00b5g per lane, probed with bsm-51432M VAMP8 (1414CT354.12.23.93) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with VAMP8 (14C3) Monoclonal Antibody (bsm-51432M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Human THP-1 cell lysates; Lane 2: Human HeLa cell lysates probed with VAMP8 Monoclonal Antibody, Unconjugated (bsm-51432M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51432M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: SH-SY5Y Cell lysates; probed with EIF2AK2 (1C4) Monoclonal Antibody, unconjugated (bsm-51436M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with EIF2AK2 (1C4) Monoclonal Antibody (bsm-51436M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).

Catalog #1: bsm-51436M

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51438M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10846M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-10846M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-10846M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Jurkat cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
HepG2 cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33037M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33037M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33037M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33037M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-33037M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33037M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33037M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-33193M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-33193M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Formalin-fixed and paraffin embedded rat kidney labeled with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33193M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-33193M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bsm-33193M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bsm-33193M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bsm-33193M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: FITC

Catalog #1: bsm-33219M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Biotin

Catalog #1: bsm-33219M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

BRL-3A cell lysates probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NIH\/3T3 Cell lysates; Lane 2: RAW246.7 Cell lysates; probed with PI3K p85 (5C11) Monoclonal Antibody, unconjugated (bsm-33219M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Human Jurkatcell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Heart lysates; Lane 6: Rat Heart lysates; Lane 7: Mouse Skeletal muscle lysates; Lane 8: Rat Skeletal muscle lysates; Lane 9: Mouse Spleen lysates; Lane 10: Human MOLT-4 cell lysates probed with PI3K p85 Monoclonal Antibody, Unconjugated (bsm-33219M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33219M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bsm-33293M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bsm-33219M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy3

Catalog #1: bsm-33219M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5

Catalog #1: bsm-33219M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy7

Catalog #1: bsm-33219M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: FITC

Catalog #1: bsm-33293M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

293T lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A549 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSK3B (3A6) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bsm-33293M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bsm-33293M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bsm-33293M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bsm-33293M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bsm-33293M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

HeLa cell lysates; probed with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cell lysates; probed with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-33042M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33042M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33042M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33042M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33042M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33042M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33042M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33187M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

A431 cell lysate probed with ACTA2 (3F9) Monoclonal Antibody (bsm-33187M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:400 overnight at 4\u00b0C, followed by incubation with a secondary antibody, DAB and counter-staining.
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Small intestine lysates; Lane 6: Mouse NIH\/3T3 cell lysates; Lane 7: Rat Stomach lysates; Lane 8: Rat Uterus lysates; Lane 9: Human A549 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human A431 cell lysates; Lane 12: Human HepG2 cell lysates probed with ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33187M

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated