Cytokeratin 17 (1B12) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded mouse prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 17 (1B12) Monoclonal Antibody (bsm-52057R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 17 (1B12) Monoclonal Antibody (bsm-52057R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$PC-3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 17 (1B12) Monoclonal Antibody (bsm-52057R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.$

PC-3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 17 (1B12) Monoclonal Antibody (bsm-52057R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52057R
Product Name	Cytokeratin 17 (1B12) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cytokeratin 17 protein, around N-terminal 100aa.
Clonality	Monoclonal
Clone #	1B12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3872
Swiss Prot	Q04695
Synonyms	39.1; CK 17; Cytokeratin17; K17; Keratin 17; Keratin type I cytoskeletal 17; Keratin17; KRT 17; KRT17; KRT17 protein; PC; PC2; PCHC1; K1C17_HUMAN.
Background	The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008].
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200