ORC2 (5C3) Monoclonal Antibody

$RIPA cell lysates from HeLa cells (30 \u03bcg) were analysed by Western blot using ORC2 (5C3) Monoclonal Antibody (bsm-53025M) diluted 1:500 in PBS-Tween containing 10% milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left.$

RIPA cell lysates from HeLa cells (30 \u03bcg) were analysed by Western blot using ORC2 (5C3) Monoclonal Antibody (bsm-53025M) diluted 1:500 in PBS-Tween containing 10% milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left.

Applications

WB
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-53025M
Product Name	ORC2 (5C3) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Monoclonal antibody raised in mouse against human ORC2, using two recombinant proteins containing the N-terminal and C-terminal part of the protein, respectively
Clonality	Monoclonal
Clone #	5C3
Isotype	IgG1
Concentration	0.6ug/ul
Purification	Protein G purified monoclonal antibody in PBS containing 0.05% azide.
Storage Buffer	PBS with 0.05% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4999
Swiss Prot	Q13416
Synonyms	ORC2L, Origin recognition complex subunit 2, ORC2
Background	Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200