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H3K9un (7D7) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-53024M
Product Name H3K9un (7D7) Monoclonal Antibody
Applications WB, IHC-P, IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Monoclonal antibody raised in mouse against a region of histone H3 containing the unmodified lysine 9 (H3K9un), using a KLH-conjugated synthetic peptide.
Clonality Monoclonal
Clone # 7D7
Isotype IgG1
Concentration 2ug/ul
Purification Protein A purified monoclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300
Storage Buffer PBS with 0.05% sodium azide and 0.05% Proclin300.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 8350
Swiss Prot P68431
Background Histones are present in the chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called histone code. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200