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Cancer


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Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Raw264.7 Cells (30ug); Lane 2: Mouse Liver (40ug); Probed with IL-1 Beta Polyclonal Antibody, unconjugated (bs-6319R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Raw264.7 cell lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1 Beta Polyclonal Antibody(bs-6319R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6319R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bcl-2) polyclonal Antibody, Unconjugated (bs-4563R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-4563R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-6319R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: HRP

Catalog #1: bs-0468R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: AbBy Fluor® 647

Formalin-fixed and paraffin embedded rat kidney labeled with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33193M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-6319R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0127R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Zebrafish

Conjugate: PerCP

Catalog #1: bs-3759R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: APC

Catalog #1: bs-6319R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy5

Catalog #1: bs-6319R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Biotin

Catalog #1: bs-4563R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: Cy3

Catalog #1: bs-4563R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: Cy5

Catalog #1: bs-4563R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: Cy7

Catalog #1: bs-4563R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: FITC

Catalog #1: bs-4563R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-4563R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-6319R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy3

Catalog #1: bs-6319R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy7

Catalog #1: bs-6319R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: FITC

Catalog #1: bsm-33193M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bsm-33193M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bsm-33193M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bsm-33193M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: FITC

Catalog #1: bsm-33193M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-33193M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Catalog #1: bs-0013R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: PE

Catalog #1: bs-0737R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: PE

Catalog #1: bs-1275R-Cy5.5

Applications: FCM

Reactivity: Mouse, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0465R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: Cy5.5

Catalog #1: bs-0468R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0737R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Others

Conjugate: Cy5.5

Catalog #1: bs-0876R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy5.5

Catalog #1: bs-0081R-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Cy5.5

Catalog #1: bs-0086R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: Cy5.5

Catalog #1: bs-0646R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Goat

Conjugate: Cy5.5

Catalog #1: bs-1640R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-2081R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat, Rabbit, Horse

Conjugate: Cy5.5

Catalog #1: bs-2130R-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-3759R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0013R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0127R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Zebrafish

Conjugate: Cy5.5

Catalog #1: bs-0415R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0754r-cy55-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-1531R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-2637R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Others

Conjugate: Cy5.5

Catalog #1: bs-4563R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Rabbit

Conjugate: Cy5.5

Catalog #1: bsm-33193M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5.5

Catalog #1: bs-0081R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: PE