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Human jurkat cells probed with MISRII\/AMHR2 Polyclonal Antibody, Unconjugated (bs-12414R) (green) at 1:20 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
293T cell lysates probed with AMHR2 Polyclonal Antibody, Unconjugated (bs-12414R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates probed with AMHR2 Polyclonal Antibody, Unconjugated (bs-12414R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12414R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43109R

Applications: ELISA, IHC-P

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-75372c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-60272c-2-mg-solid

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75853c-50mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75090c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75091c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75844c-25mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-80472C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82455c-10-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11070R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse placenta labeled with Rabbit Anti-AMIGO2 Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with AMIGO2 Polyclonal Antibody, Unconjugated (bs-11450R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (AMIGO2) Polyclonal Antibody, Unconjugated (bs-11450R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-11450R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-75858c-1g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75859c-5g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-12392R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6019R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-12307R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82502c-1-g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75854c-25mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-11039R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75489c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-80139C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82135c-250-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75886c-10mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-7960R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-75228c-500ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75230c-500ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75863c-250-mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2077R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9517R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2409R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon cancer labeled with Anti Amphiregulin Polyclonal Antibody, Unconjugated (bs-3847R) at 1:600 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded mouse fetal liver labeled with Rabbit Anti-Amphiregulin Polyclonal Antibody, Unconjugated(bs-3847R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Catalog #1: bs-3847R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Guinea Pig

Conjugate: Unconjugated

Catalog #1: bs-80512C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82490c-1-g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-71087R

Applications: ELISA/RIA

Reactivity: Animal

Conjugate: Unconjugated

Catalog #1: bs-75869c-5g

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Lane 1: Hela cell; Lane 2: HepG2 cell; Lane 3: MCF-7 cell; Probed with AMPK alpha 1 (8A1) Monoclonal Antibody (bsm-52423R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with AMPK alpha 1 (8A1) Monoclonal Antibody (bsm-52423R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with AMPK alpha 1 (8A1) Monoclonal Antibody (bsm-52423R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52423R

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Polyclonal Antibody, Unconjugated (bs-8813R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebral cortex lysates probed with AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Polyclonal Antibody, Unconjugated (bs-8813R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8813R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha 1 (Ser356) Polyclonal Antibody, Unconjugated (bs-14318R) at 1:400 overnight at 4\u00b0C, DAB staining.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-AMPK alpha 1 (Ser356) Polyclonal \r\n Antibody(bs-14318R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-14318R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

293T Cell lysates; probed with AMPK alpha 1(S496) (11G1) Monoclonal Antibody (bsm-52132R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Human Hela cell lysates; Lane 2: Human Huvec cell lysates probed with AMPK alpha 1 (Ser496) (11G1) Monoclonal Antibody, Unconjugated (bsm-52132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52132R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12972R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-13401R

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Mouse kidney and heart lysates probed with AMPK alpha 2 Polyclonal Antibody, unconjugated (bs-5575R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-AMPK alpha 2 (Ser173) Antibody(bs-5575R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-5575R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HL-60 cell lysates probed with AMPK alpha 2 Monoclonal Antibody, Unconjugated (bsm-51482M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: 293 cell lysates probed with AMPK alpha 2 Monoclonal Antibody, Unconjugated (bsm-51482M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51482M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti PRKAA2\/AMPK alpha 2 Polyclonal Antibody, Unconjugated (bs-2771R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-2771P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human kidney labeled with Anti-PRKAA2\/AMPK alpha 2 Polyclonal Antibody, Unconjugated (bs-2771R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human 293T cell lysates probed with AMPK alpha 2 Polyclonal Antibody, Unconjugated (bs-2771R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2771R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-22269R

Applications: IHC-P

Reactivity: Rat

Conjugate: Unconjugated

hepg2 lysates probed with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 (Thr198) Polyclonal Antibody, Unconjugated (bs-5551R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5551r-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Lane1: HeLa lysates; Lane 2: HepG2 lysates; Lane 3: mouse placenta lysates probed with AMPK alpha-1 Polyclonal Antibody, Unconjugated (bs-33338M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK alpha-1 Monoclonal Antibody, Unconjugated (bsm-33338M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Liver lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates probed with AMPK alpha-1 Monoclonal Antibody, Unconjugated (bsm-33338M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at -20\u2103 for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (AMPK alpha-1) monoclonal Antibody, Unconjugated (bsm-33338M) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33338M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated