Human MYH1 Ready-To-Use IHC Kit

Cat. No.: IHC0208H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

Myosin is a protein which is known to interact with actin in muscle and non-muscle cells. It contains two identical heavy chains and four light chains. Myosin molecules consist of two major regions: tails (rods) and heads. They aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. They are designated at type I (slow twitch) and type II (fast twitch).

Synonyms

A530084A17Rik, HEL71, IId, IId/x, MdMs, MHC-2X/D, MHC2X/D, MYH1, MYHC, MyHC-IId/x, MYHC-IIX, Myhs-f, Myhs-f2, Myhsf2, heavy chain Myosin, adult 1, MGC133384, Myh 1, Myh1, MYH1_HUMAN, Myosin heavy chain, Myosin heavy chain IIx/d, Myosin heavy chain skeletal muscle adult 1, Myosin light chain 1 alkali skeletal fast, Myosin-1, Myosin1, skeletal muscle, MYHa, MyHC-2x, MyHC-2X/D, MYHSA1, MYL1, Myosin-1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, MyHC-IIx/d.

Materials Supplied

Number	Component	50T	Concentration	Storage
1	PBS Buffer (powder)	2L x 2	20x	RT
2	Antigen Retrieval Buffer	20ml	100x	2-8°C
3	Endogenous Peroxidase Blocking Buffer	3ml	RTU	2-8°C
4	Blocking Buffer	3ml	RTU	2-8°C
5	Primary Antibody (Human MYH1 Rabbit pAb)	6ml	RTU	2-8°C
6	Secondary Antibody (Goat Anti-Rabbit IgG H&L / HRP)	6ml	RTU	2-8°C
7	Chromogen Component A	0.3ml	RTU	-20°C
8	Chromogen Component B	0.3ml	RTU	-20°C
9	Counter Staining Reagent	5ml	RTU	RT
10	Differentiation Reagent	6ml	RTU	RT
11	Mounting Media	5ml	RTU	RT
12	Control slide (Human skeletal muscle)	1 slide	RTU	RT
13	Datasheet	1 copy

Notes

1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
2. Do not allow slides to dry at any time during this procedure.
3. Please don't replace the matching reagents in this product with other manufacturers' products.
4. As DAB is a carcinogen, please take necessary precautions.
5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.