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Human Monoamine Oxidase B Ready-To-Use IHC Kit

Immunohistochemical analysis of paraffin embedded human liver tissue slide using IHC0196H (Human Monoamine Oxidase B IHC Kit).
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma tissue slide using IHC0196H (Human Monoamine Oxidase B IHC Kit).
Immunohistochemical analysis of paraffin embedded human brain tissue slide using IHC0196H (Human Monoamine Oxidase B IHC Kit).
Immunohistochemical analysis of paraffin embedded human kidney tissue slide using IHC0196H (Human Monoamine Oxidase B IHC Kit).

Human Monoamine Oxidase B Ready-To-Use IHC Kit

Cat. No.: IHC0196H
Sample Type:
FFPE tissue
Size:
50T (including a control slide)
Storage and Stability:
Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

MAOB (Monoamine Oxidase B), also called MAO, BRAIN, AMINE OXIDASE (FLAVIN-CONTAINING) B, is a protein that in humans is encoded by the MAOB gene. MAOB is a member of the flavin monoamine oxidase family. And it is mapped on Xp11.3. MAOB catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. Like MAOA, it also degrades dopamine. MAO-B is involved in the breakdown of dopamine, a neurotransmitter implicated in reinforcing and motivating behaviors as well as movement. MAO-B inhibition is, therefore, associated with enhanced activity of dopamine, as well as with decreased production of hydrogen peroxide, a source of reactive oxygen species.

Synonyms

6330414K01Rik; Adrenalin oxidase, Amine oxidase (flavin containing), Amine oxidase [flavin-containing] B, AOFB_HUMAN, MAO, brain, MAO, platelet, MAO-B, MAOB, MGC26382, Monoamine oxidase B, Monoamine oxidase type B.

Materials Supplied

Number Component 50T Concentration Storage
1 PBS Buffer (powder) 2L x 2 20x RT
2 Antigen Retrieval Buffer 20ml 100x 2-8°C
3 Endogenous Peroxidase Blocking Buffer 3ml RTU 2-8°C
4 Blocking Buffer 3ml RTU 2-8°C
5 Primary Antibody (Human Monoamine Oxidase B Rabbit pAb) 6ml RTU 2-8°C
6 Secondary Antibody (Goat Anti-Rabbit IgG H&L / HRP) 6ml RTU 2-8°C
7 Chromogen Component A 0.3ml RTU -20°C
8 Chromogen Component B 0.3ml RTU -20°C
9 Counter Staining Reagent 5ml RTU RT
10 Differentiation Reagent 6ml RTU RT
11 Mounting Media 5ml RTU RT
12 Control slide (Human kidney) 1 slide RTU RT
13 Datasheet 1 copy

Notes

  1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
  2. Do not allow slides to dry at any time during this procedure.
  3. Please don't replace the matching reagents in this product with other manufacturers' products.
  4. As DAB is a carcinogen, please take necessary precautions.
  5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.


As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.