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Stat1 Antibody

Applications

  • WB
  • IP
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-70684M
Product Name Stat1 Antibody
Applications WB, IP, ICC
Specificity The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human Jurkat and A431 cells.
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M249) was generated from a recombinant protein from human Stat1. This sequence has high homology to the conserved region in rat and mouse stat1 a and b.
Clonality Monoclonal
Clone # M249
Isotype IgG1
Purification Purified by Protein A.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P42224
Synonyms Stat1a, Stat1b, ISGF3, Stat1alpha, Stat1beta
Background The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.
Application Dilution
WB 1:300-5000
IP 1-2ug
ICC 1:100-500