| Overview |
| bsm-70663M |
| SHP2 (N-terminal region) Antibody |
| WB |
| The antibody detects a 72 kDa* protein in human A431 and Jurkat cells, and mouse brain. This antibody does not cross-react with SHP1. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Mouse |
| Clone (M163) was generated from a recombinant protein containing amino acids in the N-terminal region of human SHP2. This sequence is highly conserved in rat and mouse SHP2. |
| Monoclonal |
| M163 |
| IgG1 |
| Purified by Protein A. |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| Q06124 |
| PTP1D, SHPTP2, Syp |
| SHP2 (PTP1D, SH-PTP2, or Syp) is a widely expressed protein-tyrosine phosphatase (PTP) that maintains phosphotyrosine homeostasis during growth factor, cytokine, hormone and antigen receptor signaling. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP2 associates with EGF and PDGF growth factor receptors and is activated after stimulation of these receptors. Activation of SHP-2 and its association with Gab1 is critical for sustained ERK activation downstream of both growth factor and cytokine receptors. In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites. Thus, SHP2 is critical for maintaining phosphotyrosine homeostasis in many cell signaling pathways |
| Application Dilution |
| WB |
1:300-5000 |
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