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PTP1B (C-terminal region) Antibody

Applications

  • WB
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70631M
Product Name PTP1B (C-terminal region) Antibody
Applications WB, ICC
Specificity This antibody detects a 50 kDa* band corresponding to PTP1B in western blots of human A431, Jurkat, and HeLa cells. It does not detect PTP1B in rat PC12 or mouse SYF cells.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone M234 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PTP1B.
Clonality Monoclonal
Clone # M234
Isotype IgG2a
Purification Purified by Protein A.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P18031
Synonyms PTPN1; TCPTP/PTPN2
Background PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate β-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.
Application Dilution
WB 1:300-5000
ICC 1:100-500