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p38α MAP Kinase (C-terminal) M138 Antibody

Applications

  • WB
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-70583M
Product Name p38α MAP Kinase (C-terminal) M138 Antibody
Applications WB, ICC
Specificity This antibody detects a 38 kDa* protein corresponding to the apparent molecular mass of p38α on SDS-PAGE immunoblots of human Jurkat, A431, and HeLa cells.
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone M138 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of rat p38α. This sequence is identical in human and mouse p38α.
Clonality Monoclonal
Clone # M138
Isotype IgG1
Purification Purified by Protein A.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P70618
Synonyms MAPK, p38, p38alpha, p38MAPK
Background p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.
Application Dilution
WB 1:300-5000
ICC 1:100-500