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Fascin (clone 55K2) Antibody

Applications

  • WB
  • IHC
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-70496M
Product Name Fascin (clone 55K2) Antibody
Applications WB, IHC, ICC
Specificity The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of human A431 and HeLa, as well as mouse C2C12 and rat PC12.
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (55K2) was generated from full-length human fascin purified from HeLa cells. This sequence has high homology to rat and mouse fascin.
Clonality Monoclonal
Clone # 55K2
Isotype IgG1
Purification Purified by Protein A.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot Q16658
Synonyms p55
Background Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.
Application Dilution
WB 1:300-5000
IHC
ICC 1:100-500