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CD147/Emmprin/Basigin (Extracellular region) Antibody

Applications

  • WB
  • IP
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70409M
Product Name CD147/Emmprin/Basigin (Extracellular region) Antibody
Applications WB, IP, ICC
Specificity Clone M036 mouse monoclonal antibody detects a 38-58 kDa* protein on SDS-PAGE immunoblots of human BEAS, NCIH28, NCI-H1299, LNCaP, and A431 cells, as well as human brain tissues. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone (M036) was generated from a proprietary antigen related to the native human CD147 expressed in NCI-H1915 lung cancer cell line.
Clonality Monoclonal
Clone # M036
Isotype IgG2a
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P35613
Synonyms Basigin, 5F7, Collagenase stimulatory factor Extracellular matrix metalloproteinase,EMMPRIN, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, TCSF, CD147, BSG
Background D147 (Basigin/EMMPRIN) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. CD147 is a glycosylated protein with four known isoforms with isoform 2 being the most abundantly expressed. In many cancers, CD147 is overexpressed and stimulates the secretion of extracellular matrix metalloproteinases by tumor fibroblasts, which can promote tumor progression. CD147 is co-expressed with the amino acid transporter, CD98h, in metabolically active cells such as lymphocytes, macrophages, and tumor cells. CD147 interaction with caveolin and cyclophilins regulates its functions, and this protein may function outside of tumor cells through cell shedding and microvesicle release.
Application Dilution
WB 1:300-5000
IP 1-2ug
ICC 1:100-500