CD99 (Extracellular region) Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-70408M
Product Name	CD99 (Extracellular region) Antibody
Applications	WB, IP, ICC
Specificity	Clone M030 detects 30 and 32 kDa* bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation. In addition, the antibody labels live, unfixed MeWo cells.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Clone M030 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.
Clonality	Monoclonal
Clone #	M030
Isotype	IgG1
Purification	Purified by Protein G.
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot	P14209
Synonyms	CD99 antigen, 12E7, E2 antigen, MIC2 T-cell surface glycoprotein E2, MIC2X, MIC2Y
Background	The glycosylated transmembrane protein CD99 is involved in many essential cellular functions including cell adhesion, migration, cell death, differentiation, and intracellular protein trafficking. The CD99 gene encodes two distinct proteins, type I is 32 kDa and type II is 28 kDa, which are a result of the alternative splicing of the cytoplasmic region. These CD99 isoforms are expressed in a cell-type-specific manner and may have distinct functions. CD99 is overexpressed in several types of sarcomas, lymphomas, gliomas, neuroendocrine tumors, and some breast cancers. In these tumors, CD99 may have oncogenetic functions that promote migration, invasion, and metastasis of tumor cells. However, other neoplasms, carcinomas, and sarcomas have CD99 expression in benign or early-stage tumors, but lower expression in the advanced-stage counterparts. In these tumors, CD99 may have oncosuppressor signaling, and its re-expression can lead to the reversal of malignancy. Thus, CD99 is an important membrane protein involved in many aspects of cell migration and adhesion in normal and diseased cells.
Application Dilution
WB	1:300-5000
IP	1-2ug
ICC	1:100-500