New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

CD44 (Extracellular region) Antibody

Applications

  • WB
  • IP
  • ICC

Reactivity

  • Human
Overview
Catalog # bsm-70396M
Product Name CD44 (Extracellular region) Antibody
Applications WB, IP, ICC
Specificity Clone M010 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of native and denaturing human MDA-MB-231, A549, and A431 cell lysates. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemical labeling and immunoprecipitation. In addition, the antibody labels live unfixed A549 and MeWo cells, so the antibody may be useful for isolating CD44+ cells and for CD44 functional assays.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Clone M010 was generated from a proprietary antigen related to the extracellular region of human CD44.from the A431 epidermoid carcinoma cell line.
Clonality Monoclonal
Clone # M010
Isotype IgG2b
Purification Purified by Protein G.
Storage Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Target
Swiss Prot P16070
Synonyms Epican, ECMR-III, PGP-1, LHR, MDU2, MDU3, MIC4 Heparan sulfate proteoglycan HUTCH-I GP90, CD44
Background Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.
Application Dilution
WB 1:300-5000
IP 1-2ug
ICC 1:100-500