GLK Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with GLK monoclonal antibody, unconjugated (bsm-62241R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with GLK monoclonal antibody, unconjugated (bsm-62241R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-62241r-100ul
Product Name	GLK Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GCK
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2645
Swiss Prot	P35557
Subcellular location	Cytoplasm, Nucleus
Synonyms	GCK; GK; GLK; Glucokinase; HHF3; HK IV; HK4; HKIV; HXKP; LGLK; MODY2.
Background	Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen.
Application Dilution
WB	1:300-5000