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METTL3 Recombinant Antibody

Western blot analysis of (1) 293 cell lysate; (2) RAW264.7 cell lysate. Using METTL3 (bsm-62004R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Western blot analysis of (1) 293 cell lysate; (2) RAW264.7 cell lysate. Using METTL3 (bsm-62004R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62004r-100ul
Product Name METTL3 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human METTL3
Clonality Monoclonal
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 56339
Swiss Prot Q86U44
Subcellular location Nucleus
Synonyms IME4; M6A; Methyltransferase like protein 3; METTL3; MTA70; N6 adenosine methyltransferase 70 kDa subunit.
Background The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IP 1-2ug
IF
Flow-Cyt 1ug/Test
ICC/IF 1:50-100

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