UCP1/3 Recombinant Antibody

$Western blot analysis of mouse skeletal muscle cell lysate. Using UCP1\/3 (bsm-61899R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of mouse skeletal muscle cell lysate. Using UCP1\/3 (bsm-61899R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

$25 ug total protein per lane of various lysates (see on figure) probed with UCP1\/3 monoclonal antibody, unconjugated (bsm-61899R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with UCP1\/3 monoclonal antibody, unconjugated (bsm-61899R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Mouse
Rat

Overview
Catalog #	bsm-61899r-100ul
Product Name	UCP1/3 Recombinant Antibody
Applications	WB, IP
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse UCP1/3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22227
Swiss Prot	P12242,P56501
Subcellular location	Cytoplasm
Synonyms	UCP1; UCP3.
Background	Mitochondrial transporter that functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP.
Application Dilution
WB	1:300-5000
IP	1-2ug