Cullin 3 Recombinant Antibody

$Western blot analysis of SH-SY5Y cell lysate. Using Cullin 3 (bsm-61697R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of SH-SY5Y cell lysate. Using Cullin 3 (bsm-61697R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61697r-100ul
Product Name	Cullin 3 Recombinant Antibody
Applications	WB, FCM, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cullin 3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8452
Swiss Prot	Q13618
Subcellular location	Cytoplasm, Nucleus
Synonyms	cul3; Cullin3; PHA2E.
Background	Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IP	1-2ug