CCBR1 Recombinant Antibody

$Western blot analysis of (1) HepG2 cell lysate; (2) Mouse brain lysate. Using CCBR1 (bsm-61613R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HepG2 cell lysate; (2) Mouse brain lysate. Using CCBR1 (bsm-61613R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

$25 ug total protein per lane of various lysates (see on figure) probed with CCBR1 monoclonal antibody, unconjugated (bsm-61613R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CCBR1 monoclonal antibody, unconjugated (bsm-61613R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IP
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61613r-100ul
Product Name	CCBR1 Recombinant Antibody
Applications	WB, IP, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human xCT
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23657
Swiss Prot	Q9UPY5
Subcellular location	Cell membrane
Synonyms	CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT.
Background	Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane.Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress.
Application Dilution
WB	1:300-5000
IP	1-2ug
ICC/IF	1:50-100