HEC1 Recombinant Antibody

$Western blot analysis of Jurkat cell lysate. Using HEC1 (bsm-61551R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Jurkat cell lysate. Using HEC1 (bsm-61551R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61551r-100ul
Product Name	HEC1 Recombinant Antibody
Applications	WB, IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Kinetochore protein NDC80 homolog
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10403
Swiss Prot	O14777
Subcellular location	Nucleus
Synonyms	HEC; HsHec1; hsNDC80; Kinetochore protein Hec1; Kinetochore-associated protein 2; KNTC2; ndc80; TID3.
Background	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.
Application Dilution
WB	1:300-5000
IP	1-2ug