CHD4 Recombinant Antibody

$Western blot analysis of 293T cell lysate. Using CHD4 (bsm-61493R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of 293T cell lysate. Using CHD4 (bsm-61493R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-61493r-100ul
Product Name	CHD4 Recombinant Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CHD4
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1108
Swiss Prot	Q14839
Subcellular location	Cytoplasm, Nucleus
Synonyms	ATP dependent helicase CHD4; CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta.
Background	ATP-dependent helicase that binds and distorts nucleosomal DNA.Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:17626165, PubMed:28977666.Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200