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p53 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-60806r-100ul
Product Name p53 Monoclonal Antibody
Applications WB, IHC-P, IHC-F, IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human P53
Clonality Monoclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer PBS, Glycerol, BSA
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7157
Swiss Prot P04637
Subcellular location Cytoplasm, Nucleus
Synonyms Widespread p53; Wtp53; Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53; P53_HUMAN; TP53.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:50-500
IHC-F IHC-F=1:50-500
IF IF=1:20-50

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