STIP1 Monoclonal Antibody

$Tissue: Rat testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60759R$

Tissue: Rat testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60759R

$Tissue: human testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60759R$

Tissue: human testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60759R

$Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human Jurkat cell lysates probed with STIP1 Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A549 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human Jurkat cell lysates probed with STIP1 Monoclonal Antibody, Unconjugated (bsm-60759R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
ICC

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60759R
Product Name	STIP1 Monoclonal Antibody
Applications	WB, FCM, IHC-P, ICC
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human STIP1
Clonality	Monoclonal
Clone #	B5C7
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	PBS, Glycerol, BSA.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10963
Swiss Prot	P31948
Subcellular location	Cytoplasm, Nucleus
Synonyms	Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-organizing protein; NY REN 11 antigen; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; STIP1_HUMAN; Stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; Transformation sensitive protein IEF SSP 3521; Transformation-sensitive protein IEF SSP 3521.
Application Dilution
WB	=1:500-2000
FCM	FCM=1:100
IHC-P	IHC-P=1:100-500
ICC	ICC=1:50