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CD63 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD63) Monoclonal Antibody, Unconjugated (bsm-60749R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD63) Monoclonal Antibody, Unconjugated (bsm-60749R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
Overview
Catalog # bsm-60749r-100ul
Product Name CD63 Monoclonal Antibody
Applications WB, IHC-P, IHC-F, IF
Reactivity Mouse
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD63
Clonality Monoclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer PBS, Glycerol, BSA
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 967
Swiss Prot P08962
Subcellular location Cytoplasm, Cell membrane
Synonyms Lysosomal associated membrane protein 3; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; granulophysin; LAMP 3; LAMP3; lysosome associated membrane glycoprotein 3; CD 63; CD63 antigen (melanoma 1 antigen); CD63_HUMAN; gp55; granulophysin; LAMP-3; LIMP; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; PTLGP40; Tetraspanin-30; Tspan-30; Mast cell antigen AD1; ME491; melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; MGC72893; MLA 1; MLA1; ocular melanoma associated antigen; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30; Tumor biomarkers; Platelets markers.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500

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