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NFKB2 Recombinant Antibody

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: Jurkat, 2: Raji, 3: NIH\/3T3, 4: C6 Protein loading quantity: 20 \u03bcg Exposure time: 30 s Predicted MW: 97 kDa Observed MW: 120 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: Raji, 3: NIH/3T3, 4: C6 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 97 kDa Observed MW: 120 kDa

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60673r
Product Name NFKB2 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF, ICC/IF
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NFKB2
Clonality Recombinant
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4791
Swiss Prot Q00653
Subcellular location Nucleus
Synonyms NF_B-p100/p52; NFKB2_HUMAN; NFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10 (Lyt10); Nuclear factor NF-kappa-B p52 subunit;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:50-1:100
IF IF=1:100-500
ICC/IF ICC/IF=1:50

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