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PAX8 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PAX8) Monoclonal Antibody, Unconjugated (bsm-60632R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAX8) Monoclonal Antibody, Unconjugated (bsm-60632R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bsm-60632r-100ul
Product Name PAX8 Monoclonal Antibody
Applications IHC-P, IHC-F, IF, ICC/IF
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PAX8
Clonality Monoclonal
Isotype IgG
Concentration 1mg/ml
Purification affinity purified by Protein A
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7849
Swiss Prot Q06710
Subcellular location Nucleus
Synonyms Paired Box Gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired Domain Gene 8; PAX 8;PAX-8; PAX8_HUMAN.
Application Dilution
IHC-P =1:50-100
IHC-F IHC-F=1:400-800
IF IF=1:100-500
ICC/IF ICC/IF=1:50

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