Get 20% off all conjugated antibody purchases with code "CON-ANTIBODY2025"! Offer valid on direct orders only. Available for US customers and internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

SF1 Recombinant Antibody

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: HepG2, 3: PC-12, 4: NIH-3T3, 5: Mouse testis\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 52 kDa\r\nObserved MW: 50-75 kDa
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: HepG2, 3: PC-12, 4: NIH-3T3, 5: Mouse testis Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 52 kDa Observed MW: 50-75 kDa

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60616r
Product Name SF1 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot Q15637
Application Dilution
WB =1:500-1:2000
IHC-P IHC-P=1:100-500
IHC-F ICC/IF=1:20-1:100
IF Flow-Cyt=1:20-1:100
Flow-Cyt 1ug/Test
ICC/IF IF=0

Search our catalog of hundreds of thousands of products